PMEF-NX
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Mouse
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
mouse
Manufacturer/tradename:
Specialty Media, EmbryoMax®
제품 이름
EmbryoMax® Primary Mouse Embryonic Fibroblasts, PMEF, Neo Resistant, Strain FVB, Irradiated, Passage 3
biological source
mouse
Quality Segment
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Primary Mouse Embryo Fibroblasts, neo resistant, Strain FVB, are mitotically inactivated by treatment with irradiation and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Application
Research Category
Stem Cell Research
Stem Cell Research
Biochem/physiol Actions
Primary Mouse Embryo Fibroblasts
Packaging
5-6x106 ea
Preparation Note
Vials should be stored in the vapor phase of liquid nitrogen.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.