์ฝ˜ํ…์ธ ๋กœ ๊ฑด๋„ˆ๋›ฐ๊ธฐ
Merck

SCM013

NDiff Neuro-27 Medium Supplement (100x)

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

๋™์˜์–ด(๋“ค):

NDiff Medium Supplement, Neuro-27 Medium Supplement

์กฐ์ง ๋ฐ ๊ณ„์•ฝ ๊ฐ€๊ฒฉ์„ ๋ณด๋ ค๋ฉด ๋กœ๊ทธ์ธ๋ฅผ ํด๋ฆญํ•ฉ๋‹ˆ๋‹ค.

ํฌ๊ธฐ ์„ ํƒ

์ œํ’ˆ์ •๋ณด (DICE ๋ฐฐ์†ก ์‹œ ๋น„์šฉ ๋ณ„๋„)

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801

์ฃผ์š” ๋ฌธ์„œ

๋ชจ๋“  ๋ฌธ์„œ ๋ณด๊ธฐ
๊ธฐ์ˆ  ์„œ๋น„์Šค
๋„์›€์ด ํ•„์š”ํ•˜์‹ ๊ฐ€์š”? ์ €ํฌ ์ˆ™๋ จ๋œ ๊ณผํ•™์ž ํŒ€์ด ๋„์™€๋“œ๋ฆฌ๊ฒ ์Šต๋‹ˆ๋‹ค.
๋„์›€ ๋ฌธ์˜
๊ธฐ์ˆ  ์„œ๋น„์Šค
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๋„์›€ ๋ฌธ์˜

์ œํ’ˆ ์ด๋ฆ„

NDiff Neuro-27 Medium Supplement (100x), NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

form

liquid

manufacturer/tradename

Chemiconยฎ
Millipore

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

dry ice

Quality Level

100

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants and other factors that has been specifically developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium (Nichols J, 2006). This product can also be used to differentiate murine ES cells into post-mitotic neurons particularly via monolayer differentiation (Ying QL, 2003).

Physical form

NDiff Neuro-27 Medium Supplement is supplied as an aqueous solution. Optimal working concentration of the medium supplement must be determined for specific uses and cell types. In most instances, a 1:40-1:200 dilution is appropriate. Typically, Neuro-27 is diluted 1:100 for self renewal and neural differentiation of mouse ES cells.

Preparation Note

Maintain at -20 ยฐC until expiration date. Protect from light. Once thawed, aliquot unused portion and store at -20 ยฐC until future use. Once added to the cell culture medium, the product is stable at 4 ยฐC for four weeks.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

์ €์žฅ ๋“ฑ๊ธ‰

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

์‹œํ—˜ ์„ฑ์ ์„œ(COA)

์ œํ’ˆ์˜ ๋กœํŠธ/๋ฐฐ์น˜ ๋ฒˆํ˜ธ๋ฅผ ์ž…๋ ฅํ•˜์—ฌ ์‹œํ—˜ ์„ฑ์ ์„œ(COA)์„ ๊ฒ€์ƒ‰ํ•˜์‹ญ์‹œ์˜ค. ๋กœํŠธ ๋ฐ ๋ฐฐ์น˜ ๋ฒˆํ˜ธ๋Š” ์ œํ’ˆ ๋ผ๋ฒจ์— ์žˆ๋Š” โ€˜๋กœํŠธโ€™ ๋˜๋Š” โ€˜๋ฐฐ์น˜โ€™๋ผ๋Š” ์šฉ์–ด ๋’ค์—์„œ ์ฐพ์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

์ด ์ œํ’ˆ์„ ์ด๋ฏธ ๊ฐ€์ง€๊ณ  ๊ณ„์‹ญ๋‹ˆ๊นŒ?

๋ฌธ์„œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ์—์„œ ์ตœ๊ทผ์— ๊ตฌ๋งคํ•œ ์ œํ’ˆ์— ๋Œ€ํ•œ ๋ฌธ์„œ๋ฅผ ์ฐพ์•„๋ณด์„ธ์š”.

๋ฌธ์„œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ ๋ฐฉ๋ฌธ

Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Jennifer Nichols et al.
Methods in molecular biology (Clifton, N.J.), 329, 91-98 (2006-07-19)
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro.

๋ฌธ์„œ

Organoid Culture Frequently Asked Questions

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์˜ค๊ฐ€๋…ธ์ด๋“œ ๋ฐฐ์–‘ ์ž์ฃผ ๋ฌป๋Š” ์งˆ๋ฌธ

ํ™•๋ฆฝ๋œ 3์ฐจ์› ์„ธํฌ ๋ฐฐ์–‘ ํ”„๋กœํ† ์ฝœ์— ๋”ฐ๋ผ ์˜ค๊ฐ€๋…ธ์ด๋“œ๋ฅผ ๋ฐฐ์–‘ํ•˜๊ณ  ๋ถ„์„ํ•˜๋Š” ๋ฐฉ๋ฒ•์„ ๋ฐฐ์šฐ์„ธ์š”. ํŒ๊ณผ ์š”๋ น.

ํ”„๋กœํ† ์ฝœ

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

๊ตญ์ œ ๋ฌด์—ญ ํ’ˆ๋ชฉ ๋ฒˆํ˜ธ

SKUGTIN
SCM01304053252357329

์ž์‚ฌ์˜ ๊ณผํ•™์žํŒ€์€ ์ƒ๋ช… ๊ณผํ•™, ์žฌ๋ฃŒ ๊ณผํ•™, ํ™”ํ•™ ํ•ฉ์„ฑ, ํฌ๋กœ๋งˆํ† ๊ทธ๋ž˜ํ”ผ, ๋ถ„์„ ๋ฐ ๊ธฐํƒ€ ๋งŽ์€ ์˜์—ญ์„ ํฌํ•จํ•œ ๋ชจ๋“  ๊ณผํ•™ ๋ถ„์•ผ์— ๊ฒฝํ—˜์ด ์žˆ์Šต๋‹ˆ๋‹ค..

๊ณ ๊ฐ์ง€์›ํŒ€์œผ๋กœ ์—ฐ๋ฝ๋ฐ”๋ž๋‹ˆ๋‹ค.