646563
DL-Dithiothreitol solution
1 M in H2O
동의어(들):
(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C4H10O2S2
CAS 번호:
Molecular Weight:
154.25
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1719757
제품 이름
DL-Dithiothreitol solution, 1 M in H2O
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
form
liquid
reaction suitability
reagent type: reductant
concentration
1 M in H2O
density
1.04 g/mL at 20 °C
1.035 g/mL at 25 °C
Quality Level
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Application
DL-Dithiothreitol solution has been used:
- in western blot analysis
- as a supplement in lysis buffer to study its effects on sperm lysis
- as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
- in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds
Features and Benefits
Low toxicity and reduced odor compared to other thiol compounds.
General description
Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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