Sodium chloride

Name: Sodium chloride
Brand: SIGMA

BioUltra, Molecular Biology, ≥99.5% (AT)

동의어(들):

Salt, Halite, NaCl

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

NaCl

CAS 번호:

7647-14-5

Molecular Weight:

58.44

UNSPSC Code:

12352302

NACRES:

NA.25

PubChem Substance ID:

57652294

EC Number:

231-598-3

Beilstein/REAXYS Number:

3534976

MDL number:

MFCD00003477

Assay:

≥99.5% (AT)

Grade:

Molecular Biology

Form:

solid

Solubility:

H₂O: 1 M at 20 °C, clear, colorless

주요 문서

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InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

InChI

1S/ClH.Na/h1H;/q;+1/p-1

SMILES string

[Na+].[Cl-]

grade

Molecular Biology

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

product line

BioUltra

assay

≥99.5% (AT)

form

solid

availability

not available in (Sales restrictions may apply)

technique(s)

mass spectrometry (MS): suitable, western blot: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H₂O)

mp

801 °C (lit.)

solubility

H₂O: 1 M at 20 °C, clear, colorless

density

2.16 g/cm³ at 25 °C (77 °F)

anion traces

bromide (Br^-): ≤50 mg/kg, hexacyanoferrate(II) ([Fe(CN)₆]^4-): ≤1 mg/kg, iodide (I^-): ≤10 mg/kg, phosphate (PO₄^3-): ≤5 mg/kg, sulfate (SO₄^2-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: ≤0.01, λ: 280 nm A_max: ≤0.01

suitability

suitable for component for culture media

Quality Level

200

application(s)

genomic analysis

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General description

Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research

Application

Sodium chloride has been used:

as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry
in the preparation of Bold’s basal medium (BBM)
as a component of transcription and annealing buffer
to maintain osmotic balance and ensure the purity and stability of the isolated extracellular vesicles (EVs)
as a buffer for the lamination of lateral flow strips in the fabrication of lateral flow devices

Biochem/physiol Actions

Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.

Features and Benefits

Suitable for molecular biology and biochecmial research
Free from DNase, RNase, NICKase and protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
Tested for the presence of Anionic and Cationic traces

Other Notes

Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA; Simple salting out procedure for extracting DNA from human nucleated cells

For additional information on our range of Biochemicals, please complete this form.

Sales restrictions may apply

This product is intended for research purposes only, and it is not meant for human consumption.

저장 등급

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria (Peltigerales, Ascomycota)

Cornejo C, et al.

Bio-protocol (2015)

Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells

Kleinewietfeld M, et al.

Nature, 496(7446), 518-518 (2013)

A simple salting out procedure for extracting DNA from human nucleated cells.

S A Miller et al.

Nucleic acids research, 16(3), 1215-1215 (1988-02-11)

D.M. Wallace

Methods in Enzymology, 152, 39-39 (1987)

A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor

Kim T, et al.

Sensors, 15(3), 6469-6482 (2015)

모든 관련된 서류 보기

Sodium chloride

BioUltra, Molecular Biology, ≥99.5% (AT)

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

InChI key

InChI

SMILES string

grade

vapor pressure

product line

assay

form

availability

technique(s)

impurities

pH

mp

solubility

density

anion traces

cation traces

λ

UV absorption

suitability

Quality Level

application(s)

관련 카테고리

설명

General description

Application

Biochem/physiol Actions

Features and Benefits

Other Notes

안전성 정보

저장 등급

flash_point_f

flash_point_c

ppe

문서

시험 성적서(COA)

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