A3547
Achromopeptidase from bacteria
lyophilized powder, ≥1,000 units/mg solid
동의어(들):
ACP, Lysyl endopeptidase
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1,000 units/mg solid
Biological source:
bacterial (LYSOBACTER SP. BACTERIA M497-1)
biological source
bacterial (LYSOBACTER SP. BACTERIA M497-1)
Quality Level
form
lyophilized powder
specific activity
≥1,000 units/mg solid
mol wt
27 kDa
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Application
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.
Biochem/physiol Actions
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.
Physical form
crude powder
Other Notes
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
프로토콜
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
관련 콘텐츠
Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.
세포 용해 완충액, 단백질 추출 키트, 추출 효소, 기타 단백질 분리 및 정제를 위한 용해 강화제와 같은 세포 용해 및 단백질 추출 시약을 찾아보세요.
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed