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Merck

A7089

Accumax™ solution

sterile-filtered, suitable for cell culture

동의어(들):

Cell detachment solution

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352204
NACRES:
NA.75
MDL number:
MFCD01632904
Concentration:
1 ×

주요 문서

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기술 서비스
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도움 문의

sterility

sterile-filtered

form

solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

pH

6.8-7.8

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

관련 카테고리

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
TM=["Accumax"] solution has been used:
  • for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
  • for dissociation of retinal pigmented epithelial cells prior to passaging
  • for dissociation of islets from pancreas

Biochem/physiol Actions

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Features and Benefits

Ready-to-use sterile liquid for in vitro cell applications

Preparation Note

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.
This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

Disclaimer

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000470189
0000470189
0000483169
0000483169
0000452547

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Chronic beta-cell depolarization impairs beta-cell identity by disrupting a network of ca2+-regulated genes
Stancill JS, et al.
Diabetes, 66(8), 2175-2187 (2017)
Benjamin T Dodsworth et al.
Scientific reports, 10(1), 10542-10542 (2020-07-01)
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
Geng Z, et al.
PLoS ONE, 12(3), e0173575-e0173575 (2017)
Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells
Kaiser JP, et al.
Journal of King Saud University - Science, 25(1), 15-27 (2013)
Natalja Funk et al.
Scientific reports, 9(1), 4515-4515 (2019-03-16)
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role of LRRK2 mutations remain uncertain. Here, we investigated the role of LRRK2 in intracellular signal
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문서

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

인간 유도만능줄기세포의 체외 분화를 통한 호흡기 질환 연구 적용을 위한 기도 상피 폐 오가노이드 생성

폐 오가노이드는 인간 폐 발달 및 호흡기 질환 연구를 위한 가치 있는 3차원 모델입니다. 3dGRO™ 분화 프로토콜은 인간 iPSC로부터 4단계로 오가노이드를 생성합니다.

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