C9584
Carboxypeptidase B from porcine pancreas
lyophilized powder
동의어(들):
Peptidyl-
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biological source
Porcine pancreas
Quality Segment
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥125 units/mg protein
mol wt
34,000 Da± 600
purified by
affinity chromatography
composition
Protein, 40-70%
foreign activity
carboxypeptidase A ≤1%, chymotrypsin ≤0.1%, trypsin ≤5%
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
Application
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.
Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.
Packaging
Package size based on protein content
Physical form
Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note
Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L -arginine per min at pH 7.65 at 25 °C.
경고어
Danger
위험 코드
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 장기
Respiratory system
무슨 일이 일어나고 있는 걸까?
WGK 3
개인 보호 장비(PPE)
Eyeshields, Gloves
저장 등급
11 - Combustible Solids
인화점 (F)
No data available
인화점 (°C)
No data available