biological source
human lung (carcinoma)
packaging
tube of 5 μg 86012804-DNA-5UG, pkg of vial of cells 86012804-1VL
growth mode
Adherent
karyotype
Hypotriploid
morphology
Epithelial
products
Lecithin, High amounts of de-saturated fatty acids.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Enzymatic studies
Human A549 cell line has been used:
- to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
- to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
- to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells
Biochem/physiol Actions
Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Human Caucasian lung carcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.