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Merck

E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

동의어(들):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2, Sequestrene Na2

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C10H14N2Na2O8 · 2H2O
CAS 번호:
6381-92-6
Molecular Weight:
372.24
NACRES:
NA.31
PubChem Substance ID:
24894605
UNSPSC Code:
12352107
EC Number:
205-358-3
Beilstein/REAXYS Number:
3900609

주요 문서

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기술 서비스
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도움 문의

grade

Molecular Biology

Quality Level

300

assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%, Pb: ≤0.002%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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관련 카테고리

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

저장 등급

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000534767
0000536595
0000538845
0000538848
0000534250

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Ana Amaral et al.
Animals : an open access journal from MDPI, 11(1) (2021-01-21)
Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives
Chelant-enhanced heavy metal uptake by Eucalyptus trees under controlled deficit irrigation
Fine P et al.
The Science of the Total Environment, 493, 995-1005 (2014)
External quality assessment of methylmalonic acid and total homocysteine
Clinical Chemistry, 45, 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
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Protein Interactions

Formaldehyde cross-linking protocol detects specific protein interactions with confidence.

mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 PAGE/SDS-PAGE용 핸드캐스팅 겔

폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

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