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Merck

F0291

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

동의어(들):

Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
106096-93-9
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00081382

주요 문서

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제품 이름

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤-0.6 ng/mL

mol wt

16.0 kDa

packaging

pkg of 4X25 μg
pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

color

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

P09038

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FGF2 (2247)

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관련 카테고리

Analysis Note

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

Application

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

General description

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Physical form

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000451315
0000451315
0000195944
0000355296
0000372843

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
Maxwell C Eblaghie et al.
Developmental biology, 291(1), 67-82 (2006-01-18)
Lung development requires reciprocal epithelial/mesenchymal interactions, mediated by signaling factors such as Bmps made in both cell populations. To address the role of Bmp signaling in the epithelium, we have exploited the fact that Bmp receptor type Ia (Alk3) is
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