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Merck

F0895

Human Fibronectin

from human plasma, liquid, 1 mg/mL, suitable for cell culture

동의어(들):

Fibronectin

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75
MDL number:
MFCD00131062

주요 문서

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제품 이름

FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

biological source

human plasma

sterility

sterile

product line

BioReagent

form

liquid

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

0.1% (Solution)

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

saline: 0.05 M (Tris-buffered)

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... FN1(2335)

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관련 카테고리

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Fibronectin from human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Features and Benefits

  • Adhesive glycoprotein
  • Derived from human plasma
  • Recommended surface coverage of 1-5 μg/cm2

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000520094
0000520094
0000519308
0000519308
0000475011

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문서 라이브러리 방문

The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.
Farcas M A, et al.
BioMedical Engineering OnLine, 8(1), 30-30 (2009)
Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.
Altomare L, et al.
The International Journal of Artificial Organs, 33(8), 535-543 (2010)
Jonathan Iu et al.
Tissue engineering. Part A, 23(17-18), 1001-1010 (2017-05-10)
The intervertebral disc (IVD) is composed of nucleus pulposus (NP) surrounded by multilamellated annulus fibrosus (AF), and is located between the vertebral bodies. Current treatments for chronic neck or low back pain do not completely restore the functionality of degenerated
Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
Birkenkamp-Demtroder K, et al.
British Journal of Cancer, 105(4), 552-552 (2011)
Fibronectin: Molecular Structure, Fibrillar Structure and Mechanochemical Signaling
Dalton C J and Lemmon C A
Cell, 10(9), 2443-2443 (2021)
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Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

모두 보기

프로토콜

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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