H9539
Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
동의어(들):
Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
18
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 25 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates., indirect immunofluorescence: suitable, western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)
General description
The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.
Immunogen
synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.
Application
Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.
Biochem/physiol Actions
In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Other Notes
In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Anjan K Bongoni et al.
Transplantation direct, 5(4), e341-e341 (2019-04-18)
Complement activation plays an important role in the pathogenesis of renal ischemia-reperfusion (IR) injury (IRI), but whether this involves damage to the vasculoprotective endothelial glycocalyx is not clear. We investigated the impact of complement activation on glycocalyx integrity and renal
Tianle Sun et al.
Immunology and cell biology, 101(8), 735-745 (2023-05-31)
Coxsackievirus B3 (CVB3)-induced viral myocarditis (VMC) is characterized by immune cell infiltration and myocardial damage. High mobility group box 1 (HMGB1) is a highly conserved nuclear DNA-binding protein that participates in DNA replication, transcriptional regulation, repair response and inflammatory response
HMGB1 facilitates hypoxia-induced vWF upregulation through TLR2-MYD88-SP1 pathway
Bandana Singh, et al.
European Journal of Immunology (2016)
Yu Qiu et al.
Journal of cellular and molecular medicine, 25(17), 8244-8260 (2021-08-20)
Septic cardiomyopathy is a common complication of sepsis with high morbidity and mortality, but lacks specific therapy. This study aimed to reveal the role of circTLK1 and its potential mechanisms in septic cardiomyopathy. The in vitro and in vivo models
Ross Pirnie et al.
Chemical research in toxicology, 35(10), 1893-1902 (2022-08-04)
The high mobility group box 1 (HMGB1), which is released during acute acetaminophen (APAP) overdose, is thought to mediate a subsequent immune response, particularly hepatic infiltration of macrophages. The redox behavior of HMGB1 and the proteoforms of HMGB1 present in
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Instructions
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