conjugate
agarose conjugate
Quality Segment
form
saline suspension
extent of labeling
5-10 mg per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
capacity
≥0.1 mg/mL binding capacity (ovomucoid)
storage temp.
2-8°C
General description
Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.
Application
Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.
Physical form
Suspension in 1.0 M NaCl containing 15 mM sodium azide
Preparation Note
Conjugates are prepared from affinity purified lectin.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves