L1882

Lectin from Triticum vulgaris (wheat)

Name: Lectin from <I>Triticum vulgaris </I>(wheat)
Brand: SIGMA

agarose conjugate, saline suspension

동의어(들):

WGA, Wheat germ agglutinin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

saline suspension

주요 문서

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제품 이름

Lectin from Triticum vulgaris (wheat), agarose conjugate, saline suspension

conjugate

agarose conjugate

form

saline suspension

extent of labeling

5-10 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

capacity

≥0.1 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

Quality Level

200

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Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Biochem/physiol Actions

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

저장 등급

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves

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문서 라이브러리 방문

Interaction between the insulin receptor and its downstream effectors. Use of individually expressed receptor domains for structure/function analysis.

K Paz et al.

The Journal of biological chemistry, 271(12), 6998-7003 (1996-03-22)

A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM)

Binding sugars: from natural lectins to synthetic receptors and engineered neolectins

Arnaud J, et al.

Chemical Society Reviews, 42(11), 4798-4813 (2013)

Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.

Shohei Wakao et al.

Cellular and molecular life sciences : CMLS, 79(11), 542-542 (2022-10-07)

Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame.

문서

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

프로토콜

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear ﬂow and volumetric ﬂow rates in affinity chromatography.

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

conjugate

form

extent of labeling

matrix

matrix activation

capacity

storage temp.

Quality Level

관련 카테고리

설명

Application

Biochem/physiol Actions

General description

Physical form

Preparation Note

안전성 정보

저장 등급

wgk

ppe

문서

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