P2401
Pectinase from Rhizopus sp.
powder, 400-800 units/g solid
동의어(들):
Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-885-6
MDL number:
Specific activity:
400-800 units/g solid
form
powder
Quality Level
specific activity
400-800 units/g solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
−20°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants. Polygalacturonase is one of the highly abundant enzymes among all the pectinolytic enzymes. Pectinase has a wide range of industrial applications including, fruit juice extraction and its clarification. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.
Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants. Polygalacturonase is one of the highly abundant enzymes among all the pectinolytic enzymes. Pectinase has a wide range of industrial applications including, fruit juice extraction and its clarification. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Pectinase from Rhizopus sp has been used for enzymatic digestion of leaves.
Biochem/physiol Actions
Pectinases hydrolyses pectin, which is a component of the cell wall.They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
프로토콜
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
관련 콘텐츠
QC Methods
Isolation of intact sub-dermal secretory cavities from Eucalyptus
Goodger JQ, et al.
Plant methods, 6(1), 20-20 (2010)
Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity
Maria de Lourdes T. M. Polizeli, et al.
Microbiology, 137, 1815-1823 (1991)
Enzyme disintegration with spatial resolution reveals different distributions of sludge extracellular polymer substances
Lu Fan, et al.
Biotechnology for Biofuels, 9(1), 29-29 (2016)