P3563
Phosphate Buffered Saline
powder, pH 7.4, with 0.05% Tween® 20
동의어(들):
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25
제품 이름
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,
form
powder
Quality Level
color
white
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
application(s)
cell analysis
diagnostic assay manufacturing
유사한 제품을 찾으십니까? 방문 제품 비교 안내
General description
Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
Application
Phosphate buffered saline has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
- cell isolation
Packaging
Foil pouches
Preparation Note
Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
저장 등급
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Kossi Komlan et al.
PLoS neglected tropical diseases, 12(3), e0006312-e0006312 (2018-03-02)
Mass drug administration (MDA) of ivermectin has become the main intervention to control onchocerciasis or "river blindness". In Togo, after many years of MDA, Onchocerca volvulus infection has declined dramatically, and elimination appears achievable, but in certain river basins the
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
Modern Pathology, 22(11), 1423-1423 (2009)
프로토콜
간접 ELISA 및 포획 ELISA 실시에 관한 상세한 지침을 제공하는 ELISA 프로토콜 샌드위치 ELISA 및 간접 ELISA 실험을 실시하기 위한 권장 제품과 기법에 대해 자세히 알아봅니다.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.