sterility
aseptically filled
form
lyophilized powder
specific activity
≥2 units/mg solid
storage temp.
2-8°C
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Physical form
Lyophilized powder containing lactose
Preparation Note
Prepared from P 3026
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
저장 등급
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Henri G Gerken et al.
Planta, 237(1), 239-253 (2012-09-27)
Cell walls of microalgae consist of a polysaccharide and glycoprotein matrix providing the cells with a formidable defense against its environment. We characterized enzymes that can digest the cell wall and weaken this defense for the purpose of protoplasting or
Ross G Atkinson et al.
BMC plant biology, 12, 129-129 (2012-08-04)
While there is now a significant body of research correlating apple (Malus x domestica) fruit softening with the cell wall hydrolase ENDO-POLYGALACTURONASE1 (PG1), there is currently little knowledge of its physiological effects in planta. This study examined the effect of
Mahejibin Khan et al.
Annual review of food science and technology, 4, 21-34 (2012-11-30)
The understanding that enzymatic degradation of fruit pectin can clarify juices and improve juice yields resulted in the search for microbial pectinases and application in vegetable- and fruit-processing industries. Identified enzymes were classified on the basis of their catalytic activity