GlycoProfile^™ 2-AB Labeling Kit

The GlycoProfile^™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.

While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.

GlycoProfile^™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and human epididymis protein 4. It has also been used for labelling free oligosaccharides from mouse spleen.

Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC