์ฝ˜ํ…์ธ ๋กœ ๊ฑด๋„ˆ๋›ฐ๊ธฐ
Merck

SAE0220

Mucinase SmE Protease

cleaves between two glycosylated residues bearing complex O-linked glycans and suitable for mass spec glycoproteomics

๋™์˜์–ด(๋“ค):

SmEnhancin

์กฐ์ง ๋ฐ ๊ณ„์•ฝ ๊ฐ€๊ฒฉ์„ ๋ณด๋ ค๋ฉด ๋กœ๊ทธ์ธ๋ฅผ ํด๋ฆญํ•ฉ๋‹ˆ๋‹ค.

ํฌ๊ธฐ ์„ ํƒ

๋ณด๊ธฐ ๋ณ€๊ฒฝ

์ œํ’ˆ์ •๋ณด (DICE ๋ฐฐ์†ก ์‹œ ๋น„์šฉ ๋ณ„๋„)

NACRES:
NA.21
Recombinant:
expressed in E. coli
๊ธฐ์ˆ  ์„œ๋น„์Šค
๋„์›€์ด ํ•„์š”ํ•˜์‹ ๊ฐ€์š”? ์ €ํฌ ์ˆ™๋ จ๋œ ๊ณผํ•™์ž ํŒ€์ด ๋„์™€๋“œ๋ฆฌ๊ฒ ์Šต๋‹ˆ๋‹ค.
๋„์›€ ๋ฌธ์˜


์ œํ’ˆ ์ด๋ฆ„

Mucinase SmE, mass spec suitable, from Serratia marcescens, recombinant, liquid

recombinant

expressed in E. coli

Quality Segment

form

liquid

mol wt

98 kDa

suitability

suitable for mass spectrometry

shipped in

dry ice

storage temp.

-10 to -25ยฐC

General description

Mucinase SmE is a mucin-selective protease that has been engineered to have high activity and and tolerance for site ocupancy and glycoform complexity. Mucinase SmE provides a complementary cleavage profile to Mucinase StcE (Cat No SAE0202). Contrary to StcE digestion that requires an additional tryptic digest prior to MS peptide analysis, when using Mucinase SmE analysis can be done with SmE alone. It is recommended to perform both options to obtain maximal coverage.

Application

Mucinase SmE is useful for glycoproteomics experimentation. It is designed for digestion of densely glycosylated mucin proteins for mass spec based analyses.

Features and Benefits

  • Mucin-selective Protease
  • Cleaves N-terminally to O-link glycosylated Ser/Thr in mucin domains
  • Complimentary cleavage profile to Mucinase StcE
  • Accommodates complex, dense and sialylated glycoforms
  • Recombinant with His-Tagยฎ

Preparation Note

Thaw the Mucinase SmE by brief incubation at 37ยฐC.

The protein solution is stable for 2 weeks at 2-8 ยฐC.

For longer storage, aliquot the protein solution and store at -20 ยฐC.

The enzyme solution should be protected from direct light.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany


์ €์žฅ ๋“ฑ๊ธ‰

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available



๊ฐ€์žฅ ์ตœ์‹  ๋ฒ„์ „ ์ค‘ ํ•˜๋‚˜๋ฅผ ์„ ํƒํ•˜์„ธ์š”:

์‹œํ—˜ ์„ฑ์ ์„œ(COA)

Lot/Batch Number

์ ํ•ฉํ•œ ๋ฒ„์ „์„ ์ฐพ์„ ์ˆ˜ ์—†์œผ์‹ ๊ฐ€์š”?

ํŠน์ • ๋ฒ„์ „์ด ํ•„์š”ํ•œ ๊ฒฝ์šฐ ๋กœํŠธ ๋ฒˆํ˜ธ๋‚˜ ๋ฐฐ์น˜ ๋ฒˆํ˜ธ๋กœ ํŠน์ • ์ธ์ฆ์„œ๋ฅผ ์ฐพ์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

์ด ์ œํ’ˆ์„ ์ด๋ฏธ ๊ฐ€์ง€๊ณ  ๊ณ„์‹ญ๋‹ˆ๊นŒ?

๋ฌธ์„œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ์—์„œ ์ตœ๊ทผ์— ๊ตฌ๋งคํ•œ ์ œํ’ˆ์— ๋Œ€ํ•œ ๋ฌธ์„œ๋ฅผ ์ฐพ์•„๋ณด์„ธ์š”.

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