T2507
Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)
Type I, ammonium sulfate suspension, ~10,000 units/mg protein
동의어(들):
D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
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제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
Specific activity:
~10,000 units/mg protein
type
Type I
Quality Segment
form
ammonium sulfate suspension
specific activity
~10,000 units/mg protein
mol wt
53 kDa by equilibrium centrifugation
foreign activity
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
storage temp.
2-8°C
Application
Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.
Physical form
Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)