Tetanolysin from Clostridium tetani

Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.

Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)¹. It has also been used to permeabilize infected RBCs².

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell..

Prepared by a modification of the method of Haque, et al.

When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.

Single band by SDS-PAGE.