T8154

Trypan Blue solution

Name: Trypan Blue solution
Brand: SIGMA

0.4%, liquid, sterile-filtered, suitable for cell culture

동의어(들):

Blue cell viability dye

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₃₄H₂₄N₆Na₄O₁₄S₄

CAS 번호:

72-57-1

Molecular Weight:

960.81

UNSPSC Code:

12352207

NACRES:

NA.75

PubChem Substance ID:

57654704

MDL number:

MFCD00003969

Beilstein/REAXYS Number:

4360496

주요 문서

모든 문서 보기

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

제품 이름

Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture

form

liquid

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

sterility

sterile-filtered

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

Quality Level

200

유사한 제품을 찾으십니까? 방문 제품 비교 안내

Application

Trypan Blue solution has been used:

in cell viability assay to count viable and dead cells
in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer

General description

For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

저장 등급

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The sensitivity of 5-formylcytosine to doxorubicin regardless of DNA damage

Selcen

Turkiye Entomoloji Dergisi (Turkish Journal of Entomology), 40 (2016)

Single cell isolation process with laser induced forward transfer

Yu Deng

Journal of Biological Engineering, 11(2) (2017)

Vital dyes in vitreoretinal surgery: chromovitrectomy

Meyer CH

Developments in Ophthalmology, 42 (2008)

Roya Khosravi-Far, Zahra Zakeri

Programmed Cell Death (2008)

The tumor suppressor PTEN is exported in exosomes and has phosphatase activity in recipient cells.

Ulrich Putz et al.

Science signaling, 5(243), ra70-ra70 (2012-09-27)

Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipids, DNA, or microRNAs) can alter the physiological states of recipient cells. We demonstrated that phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor

모든 관련된 서류 보기

문서

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Cell Counting Using a Hemocytometer

Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.

세포 생존력 및 증식 분석

암, 신경과학 및 줄기세포 연구에 응용할 수 있는 세포 증식(BrdU, MTT, WST1), 세포 생존력 및 세포 독성 실험을 위한 세포 기반 분석입니다.

프로토콜

유도만능줄기세포 배양 프로토콜

iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

Subculture of Adherent Cell Lines

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

모두 보기

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

form

InChI

SMILES string

InChI key

sterility

storage condition

concentration

technique(s)

application(s)

shipped in

Quality Level

관련 카테고리

설명

Application

General description

Preparation Note

안전성 정보

pictograms

signalword

hcodes

pcodes

Hazard Classifications

저장 등급

wgk

flash_point_f

flash_point_c

ppe

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

관련 참고 프로토콜

문서

프로토콜

관련 콘텐츠

기술 서비스