T9424
TRI Reagent®
For processing tissues, cells cultured in monolayer or cell pellets
동의어(들):
TRI Reagent® RNA Isolation Reagent
제품 이름
TRI Reagent®, For processing tissues, cells cultured in monolayer or cell pellets
usage
mL sufficient for 107 cells
mL sufficient for 100 mg tissue (or)
storage temp.
2-8°C
Quality Level
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Application
- in the isolation of total RNA from various tissue samples and cells
- for the lysis of monocytes
- to isolate cytoplasmic RNA from SARS-CoV infected Vero cells
- to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
- to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine
The RNA, DNA and protein that are isolated using TRI reagent® can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
Features and Benefits
Multi-purpose: TRI Reagent® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples.
Efficient: TRI reagent® gives better yields than traditional guanidine thiocyanate/cesium chloride methods. The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour.
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
General description
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Legal Information
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
저장 등급
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
문서
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
프로토콜
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
TRI 시약은 DNA, RNA 및 단백질을 동시에 분리할 수 있게 하는 단일 과정의 RNA 분리 시약입니다. 이 프로토콜은 시료 제조 및 다양한 시료 추출, 문제 해결의 개요를 제공합니다.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
관련 콘텐츠
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.