W1754
Water
PCR Reagent, suitable for PCR
동의어(들):
H2O
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제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
H2O
CAS 번호:
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
제품 이름
Water, PCR Reagent
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.
Application
Suitable for polymerase chain reaction (PCR)
Water has been used:
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)
Other Notes
Easily compare specifications for Water products with the Water specification table.
저장 등급
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
프로토콜
표준 PCR 프로토콜 단계를 알아보고 시약 목록 또는 사이클링 매개변수를 검토하세요. 일상적인 DNA 증폭을 위한 이 방법은 표준 Taq DNA 중합효소를 사용합니다.
SYBR Green qPCR 프로토콜은 유전자 발현 및 RT-PCR 반응의 정확한 정량화를 감지하도록 설계된 방법입니다.
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the