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제품정보 (DICE 배송 시 비용 별도)
NACRES:
NB.22
UNSPSC Code:
41122410
Manufacturer/tradename:
Excel Scientific TSS-RTQ-100
Material:
polyolefin film, silicone/adhesive
W × l:
7.62 cm × 13.34 cm
Sterility:
non-sterile
Packaging:
pack of 100 ea
material
polyolefin film, silicone/adhesive
sterility
non-sterile
packaging
pack of 100 ea
manufacturer/tradename
Excel Scientific TSS-RTQ-100
technique(s)
PCR: suitable, qPCR: suitable
L × W
76.2 mm × 133.4 mm
W × L
7.62 cm × 13.34 cm
color
clear
General description
- High optical clarity
- Minimal to no autofluorescence
- Chemically inert; no extractables except at extreme pH
- DMSO resistant for HTS
- Heat resistant, recommended for temperatures from
- -70°C to +100°C
- Certified DNase, RNase, and Nucleic Acid free
- Fit within raised plate rim to prevent loss of seal due to film lifting
- Silicone adhesive forms the strongest available seal for evaporation prevention
- Non-tacky adhesive layer simplifies handling of film prior to sealing
ThermalSeal RTS™ sealing film is a 50 μm thick polyolefin with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
Legal Information
ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RTS is a trademark of Excel Scientific, Inc.
프로토콜
SYBR Green qPCR 프로토콜은 유전자 발현 및 RT-PCR 반응의 정확한 정량화를 감지하도록 설계된 방법입니다.
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.