Silica Column Regeneration Solution

Silica Column Regeneration Solution contains a mixture of acetic acid, dimethoxypropane, and methylene chloride.

This solution effectively regenerates a silica column that has come into contact with very strongly polar solvents, such as water or alcohols. Simply flush the column with regeneration solution for 10 minutes, then reequilibrate with mobile phase for 10 minutes. Column performance usually is restored to that obtained before exposure to the polar solvent.

It was used to flush and regenerate the column used for HPLC analysis of tocopherols and tocotrienols.