201626
Fluoresceinamine, isomer I
Synonym(s):
5-Aminofluorescein
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About This Item
Empirical Formula (Hill Notation):
C20H13NO5
CAS Number:
Molecular Weight:
347.32
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
222-043-6
Beilstein/REAXYS Number:
48395
MDL number:
form
powder
Quality Level
mp
223 °C (dec.) (lit.)
solubility
methanol: 5 mg/mL
λmax
490-501 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChI key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
General description
Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.
Application
Fluoresceinamine, isomer I is suitable for use in a specific and sensitive spectrophotometric method for determining nitrite. It has been used to fluorescently tag nanoparticles through a competitive carboxyl-amine coupling reaction to visualize nanoparticle internalization.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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S Miyauchi et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 12(1), 27-34 (1996-01-01)
The protective efficacy of sodium hyaluronate (Na-HA) on the corneal endothelium against the damage induced by sonication was investigated using enucleated rabbit eyes and Na-HA fluorescence labeled with 5-aminofluorescein (FA-HA). The anterior chamber was reformed by injecting a 1% solution
F Schwesinger et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(18), 9972-9977 (2000-08-30)
Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine different single-chain Fv fragments, whose on-rates, off-rates, and equilibrium binding affinities were determined in solution. Additionally, activation energies for unbinding were estimated from the temperature dependence of the
J T Jones et al.
Molecular and biochemical parasitology, 30(1), 35-44 (1988-07-01)
We have studied the characteristics of binding of the polycation poly-L-lysine to the schistosome surface. Two consequences of this binding were measured: (a) tegumental damage, as assessed by the uptake of the DNA binding stain Hoechst 33258, and (b) the