35848
2′,7′-Dichlorofluorescein
BioReagent, suitable for fluorescence, ≥90% (T)
Synonym(s):
DCF, Dichlorofluorescein
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About This Item
Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
Molecular Weight:
401.20
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
200-968-6
Beilstein/REAXYS Number:
58009
MDL number:
product line
BioReagent
Quality Level
assay
≥90% (T)
form
solid
mp
280 °C (dec.) (lit.)
fluorescence
λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
Application
2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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W.D. Pfeffer et al.
Journal of Chromatography A, 506, 401-401 (1990)
H. Watanabe et al.
Analytical Sciences, 2, 461-461 (1986)
G Gübitz et al.
Journal of chromatography, 117(2), 337-343 (1976-02-18)
An in situ fluorimetric method has been developed for the quantitation of gluconic and lactobionic acids and their salts in tablet formulations. The method is based on glycol cleavage with lead tetraacetate followed by treatment with dichlorofluorescein. Calcium gluconate and