C4951
Cholesterol-Water Soluble
powder, BioReagent, suitable for cell culture
Synonym(s):
Cholesterol–methyl-β-cyclodextrin
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352211
biological source
ovine wool
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 300 mg/mL
shipped in
ambient
storage temp.
−20°C
Quality Level
Application
Cholesterol-Water Soluble has been used:
- in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
- in cytotoxicity assay
- for the incubation of CD4+ T cells with Helicobacter pylori
- to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
- to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
- as a supplement for growth of bacterial culture
- to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
- to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
- for reversible inhibition of proteinase K
Packaging
Package size based on cholesterol
Preparation Note
Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.
Other Notes
Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2
Glende J, et al.
Virology, 381(2), 215-221 (2008)
A novel reciprocal and biphasic relationship between membrane cholesterol and beta-secretase activity in SH-SY5Y cells and in human platelets
Liu WW, et al.
Journal of Neurochemistry, 108(2), 341-349 (2009)
Cholesterol and its derivatives reversibly inhibit proteinase K
Singh N and Bhattacharyya D
Journal of Cellular Physiology, 232(3), 596-609 (2017)
Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons
Abe K and Saito H
Neuroscience Research, 35(3), 165-174 (1999)
Cholesterol influences potassium currents in inner hair cells isolated from guinea pig cochlea
Kimitsuki T
Auris, Nasus, Larynx, 44(1), 46-51 (2017)
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