S7531
Anti-Staphylococcal α-Toxin antibody produced in rabbit
whole antiserum
Synonym(s):
Anti-α-Hemolysin
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, ELISA (i)
Citations:
28
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen 33 kDa
contains
15 mM sodium azide
species reactivity
Staphylococcus aureus
packaging
antibody small pack of 25 μL
technique(s)
dot blot: 1:20,000, indirect ELISA: 1:50,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Staphylococcal α toxin, a water soluble 33kD single polypeptide, is a cytotoxic agent secreted by Staphylococcus aureus. This protein has membrane damaging properties and accounts for erythrocyte lysis. Hence it is also referred to as lethal hemolytic toxin or α-hemolysin . Anti-staphylococcal α-toxin antibody can be used for studies of the toxin-membrane interaction. Rabbit anti-staphylococcal α-toxin antibody reacts specifically with staphylococcal α-toxin but not with staphylococcal enterotoxin A, cholera toxin or pseudomonas exotoxin A.
Immunogen
α-toxin (α-hemolysin) from Staphylococcus aureus
Application
Anti-staphylococcal α-toxin antibody can be used in indirect ELISA (1:50,000) and dot blot immunoassay (1: 20,000).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Analysis Note
The antiserum has been treated to remove lipoproteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
María Belén Mestre et al.
Autophagy, 6(1), 110-125 (2010-01-30)
Staphylococcus aureus is a pathogen that causes serious infectious diseases eventually leading to septic and toxic shock. Classically S. aureus has been considered an extracellular pathogen, but cumulative evidence indicates that it invades cells and replicates intracellularly leading to staphylococcal
Alicia Lacoma et al.
Toxins, 13(2) (2021-02-11)
Staphylococcus aureus is a commensal and frequent colonizer of the upper respiratory tract. When mechanical ventilation disrupts natural defenses, S. aureus is frequently isolated from the lower airways, but distinguishing between colonization and infection is difficult. The objectives of this
S Forti et al.
European journal of biochemistry, 181(3), 767-773 (1989-05-15)
alpha-Toxin, a lethal hemolytic toxin secreted by Staphylococcus aureus, forms ionic channels of large size in lipid membranes. To investigate the mechanism of channel assembly we have studied the kinetics of pore formation on small unilamellar vesicles. We have used