341738
Sulforhodamine B, acid form
Dye content 95 %
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About This Item
Empirical Formula (Hill Notation):
C27H30N2O7S2
CAS Number:
Molecular Weight:
558.67
NACRES:
NA.23
PubChem Substance ID:
UNSPSC Code:
12352103
EC Number:
220-025-2
MDL number:
InChI key
IOOMXAQUNPWDLL-UHFFFAOYSA-N
InChI
1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)
SMILES string
CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1
form
solid
composition
Dye content, 95%
mp
>300 °C (lit.)
λmax
558 nm
Quality Level
Related Categories
General description
Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.
Application
pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Lindsay N Sanford et al.
Analytical biochemistry, 434(1), 26-33 (2012-11-13)
Accurate control of the sample temperature during thermal cycling is critical for successful polymerase chain reaction (PCR). Direct sensor contact with the reaction is problematic, forcing measurements external to the sample and compromising accuracy during rapid temperature transitions. The widespread
Katarzyna Sokołowska et al.
American journal of botany, 99(11), 1745-1755 (2012-11-06)
The picture of how long-distance transport proceeds in trees is still far from being complete. Beside the apoplasmic pathway, transport undoubtedly also takes place within the system of living cells in the secondary xylem and cambial region. Because detailed, thorough
Fujun Han et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 81(6), 532-540 (2012-05-11)
Multiparametric image analysis is highly preferable in revealing complicated biological processes. The multiplexing capability is demanding and is limited by spectral overlap of fluorescent dyes and the number of fluorescent channels available in an imaging platform, especially when both nuclear
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