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About This Item
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
5107482
Product Name
Dihydroethidium, ≥95%
Quality Level
assay
≥95%
form
powder
technique(s)
titration: suitable
color
pink to very dark red
solubility
chloroform: 20 mg/mL
fluorescence
λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Redox indicator. Blue fluorescence until oxidized to ethidium.
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).
Packaging
Packaged under Argon.
Other Notes
B-ring reduced analog of ethidium
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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