P5504
Potassium phosphate dibasic trihydrate
ReagentPlus®, ≥99.0%
Synonym(s):
Dipotassium hydrogen phosphate trihydrate, Dipotassium phosphate, di-Potassium hydrogen phosphate trihydrate
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About This Item
Linear Formula:
K2HPO4 · 3H2O
CAS Number:
Molecular Weight:
228.22
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
231-834-5
MDL number:
Quality Level
product line
ReagentPlus®
assay
≥99.0%
form
powder or crystals
pH
9.2-9.4 (25 °C, 50 g/L)
solubility
water: 100 mg/mL, clear, colorless
storage temp.
room temp
SMILES string
O.O.O.[K+].[K+].OP([O-])([O-])=O
InChI
1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2
InChI key
XQGPKZUNMMFTAL-UHFFFAOYSA-L
General description
Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.
Application
Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Interaction between Potassium Phosphate Buffer Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy
Weilai Y, et al.
Chinese Journal of Chemical Physics, 28(4), 518-524 (2015)
Daniel Valdepérez et al.
Journal of colloid and interface science, 474, 1-8 (2016-04-19)
Polyelectrolyte hollow capsules are versatile platforms typically used for encapsulation of a wide variety of macromolecules in their cavity. The polymer shell of these capsules as composed by alternating layers of oppositely charged polyelectrolytes also allows for adding additional functionalities.
Imidazolium-based Ionic Liquids as Adjuvants to Form Polyethylene Glycol with Salt Buffer Aqueous Biphasic Systems
Santos JHPM, et al.
Journal of Chemical and Engineering Data, 65(8), 3794?3801-3794?3801 (2020)