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B6916

Bradford Reagent

Name: Bradford Reagent
Brand: SIGMA

for 0.1-1.4 mg/ml protein

Synonym(s):

Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent

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About This Item

NACRES:

NA.32

UNSPSC Code:

12161500

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Quality Level

200

form

solution

storage temp.

2-8°C

Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Application

Bradford Reagent has been used to determine total protein concentration.

Features and Benefits

The reagent is ready to use. No mixing or dilution required.
Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
Can be used in microwell plate assays.
Inexpensive assay.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

pictograms

GHS08,GHS05

signalword

Warning

hcodes

H290,H315,H319,H371

pcodes

P234 - P260 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P311

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000481629

0000362297

0000261841

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