form
lyophilized powder
Quality Level
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 4-5 mL
particle size
45—165 μm
storage temp.
2-8°C
General description
Cyanogen bromide-activated-Sepharose™ is suitable for use in affinity chromatography and protein chromatography. It has been used to discover insecticidal agents, raise polyclonal antibodies against yeast invertase glycosyls, and study immunological processes of xenograft rejection.
C9142-15G′s updated product number is GE17-0430-01
C9142-15G′s updated product number is GE17-0430-01
Application
Cyanogen bromide-activated-Sepharose™ 4B has been used:
- in the purification of clusterin from serum by an immunoaffinity-based separation procedure
- in affinity chromatography to isolate Leishmania donovani protein antigens from the urine of visceral leishmaniasis patients
- in affinity chromatography for the isolation of haptoglobin from human serum
Physical form
Lyophilized powder stabilized with lactose and dextran
Legal Information
Sepharose is a trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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