L0159
Anti-Luciferase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti Luciferase Antibody, Firefly Luciferase Antibody, Luciferase Antibody, Luciferase Antibody - Anti-Luciferase antibody produced in rabbit
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
46
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
technique(s)
indirect immunofluorescence: 1:500-1:1,000 using eukaryotic cells transfected with a plasmid bearing the luciferase gene, western blot: 1:10,000 using purified Luciferase
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Related Categories
General description
Analysis of gene expression is commonly assayed by transient transfection. These systems are usually based on the use of fusion genes which are inserted into cells, and expression of the gene is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions is presumed to reflect the ability of the sequences studied to direct or promote transcription.
Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity.
Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity.
Immunogen
firefly (Photinus pyralis) luciferase.
Application
Anti-Luciferase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
The antibody identifies recombinant luciferase in eukaryotic cells transfected with a plasmid bearing the luciferase gene.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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Marco Quarta et al.
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A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters
Fromme J Chris and Kim J
Analytical biochemistry, 421(2), 482-488 (2012)
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Intraductal delivery of adenoviruses targets pancreatic tumors in transgenic Ela-myc mice and orthotopic xenografts
Jose A, et al.
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Marco Chiabudini et al.
Molecular and cellular biology, 32(23), 4769-4779 (2012-09-26)
When a polyadenylated nonstop transcript is fully translated, a complex consisting of the ribosome, the nonstop mRNA, and the C-terminally polylysine-tagged protein is generated. In Saccharomyces cerevisiae, a 3-step quality control system prevents formation of such dead-end complexes. Nonstop mRNA
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