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About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
magnetic beads
Clone:
M2, monoclonal
Application:
IP, affinity chromatography
Citations:
1017
conjugate
magnetic beads
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
suspension
shelf life
2 yr at -20 °C
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
bead size
20-75 μm
matrix
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
isotype
IgG1
capacity
≥0.6 mg/mL binding capacity
shipped in
wet ice
storage temp.
−20°C
General description
Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.
Application
Suitable for immunoprecipitation procedures.
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Learn more product details in our FLAG® application portal.
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Learn more product details in our FLAG® application portal.
Features and Benefits
The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest
Physical form
Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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