P1644
Percoll®
pH 8.5-9.5 (20 °C)
Synonym(s):
Percoll Density Gradient Media
sterility
aseptically filled
form
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
technique(s)
cell culture | mammalian: suitable
color
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
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General description
Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.
Application
Percoll® has been used in sperm preparation to study the energy requirements of bovine embryos.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Legal Information
Percoll is a registered trademark of Cytiva
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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R Hovius et al.
Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different
Tanja Zöller et al.
International journal of molecular sciences, 19(3) (2018-03-02)
Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we
T Hirano et al.
The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (approximately 3-micron long), which runs through a metaphase plate-like structure consisting
Noa Bossel Ben-Moshe et al.
Nature communications, 10(1), 3266-3266 (2019-07-25)
Complex interactions between different host immune cell types can determine the outcome of pathogen infections. Advances in single cell RNA-sequencing (scRNA-seq) allow probing of these immune interactions, such as cell-type compositions, which are then interpreted by deconvolution algorithms using bulk
Development of bovine embryos in vitro as affected by energy substrates.
Rosenkrans Jr C F, et al.
Biology of Reproduction, 49(3), 459-462 (1993)
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