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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Phosphoriboisomerase from spinach, Type I, partially purified powder, ≥40 units/mg protein (biuret)
biological source
spinach
type
Type I
form
partially purified powder
specific activity
≥40 units/mg protein (biuret)
storage temp.
−20°C
Quality Level
Application
Phosphoriboisomerase is used to study the ribulose monophosphate pathway. Phosphoriboisomerase from spinach is used in enzyme assays to convert ribose 5-phosphate to ribulose 5-phosphate.
Biochem/physiol Actions
Phosphoriboisomerase is responsible for the converstion of ribose 5-phosphate to ribulose 5-phosphate.
Other Notes
One unit will convert 1.0 μmole of D-ribose 5-phosphate to D-ribulose 5-phosphate per min at pH 7.7 at 30 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Thomas E Edwards et al.
BMC structural biology, 11, 39-39 (2011-10-15)
Ribose-5-phosphate isomerase is an enzyme that catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate. This family of enzymes naturally occurs in two distinct classes, RpiA and RpiB, which play an important role in the pentose phosphate pathway and nucleotide and co-factor
Z Wu et al.
Journal of applied microbiology, 106(1), 41-48 (2009-01-06)
This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when cells were grown
Mirjam M C Wamelink et al.
Journal of molecular medicine (Berlin, Germany), 88(9), 931-939 (2010-05-26)
Ribose 5-phosphate isomerase (RPI) deficiency is an enzymopathy of the pentose phosphate pathway. It manifests with progressive leukoencephalopathy and peripheral neuropathy and belongs, with one sole diagnosed case, to the rarest human disorders. The single patient was found compound heterozygous
Richard W Strange et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 65(Pt 12), 1214-1217 (2010-01-08)
Ribose-5-phosphate isomerase is a ubiquitous intracellular enzyme of bacterial, plant and animal origin that is involved in the pentose phosphate cycle, an essential component of cellular carbohydrate metabolism. Specifically, the enzyme catalyses the reversible conversion of ribose 5-phosphate to ribulose
Anna Tylki-Szymańska et al.
Postepy biochemii, 57(2), 168-171 (2011-09-15)
Two previously unreported inborn errors of metabolism occur in the reversible part of the pentose phosphate pathway. Deficiency of ribose-5-phosphate isomerase has been described in one patient who suffered from a progressive leukoencephalopathy and developmental delay. Transaldolase deficiency has been
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