S7896
Syringaldazine
indicator for laccase and peroxidase activity, ≥98% (TLC), powder or crystals
Synonym(s):
4-Hydroxy-3,5-dimethoxybenzaldehyde azine
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About This Item
Linear Formula:
[HOC6H2(OCH3)2CH=N-]2
CAS Number:
Molecular Weight:
360.36
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
238-390-1
MDL number:
Product Name
Syringaldazine, indicator for laccase and peroxidase activity
Quality Level
assay
≥98% (TLC)
form
powder or crystals
mp
209-210 °C (lit.)
solubility
DMF: soluble, slightly hazy
storage temp.
2-8°C
SMILES string
COc1cc(\C=N\N=C\c2cc(OC)c(O)c(OC)c2)cc(OC)c1O
InChI
1S/C18H20N2O6/c1-23-13-5-11(6-14(24-2)17(13)21)9-19-20-10-12-7-15(25-3)18(22)16(8-12)26-4/h5-10,21-22H,1-4H3/b19-9+,20-10+
InChI key
YARKTHNUMGKMGS-LQGKIZFRSA-N
Application
Syringaldazine has been used as a substrate to determine the enzymatic activity of the cuticular laccase.
Biochem/physiol Actions
Syringaldazine is a dimer of two molecules of 2,6-dimethoxyphenol linked by an azide bridge. It is a phenolic non-autooxidizable laccase-specific compound, that facilitates the detection of fungal laccase in some varieties of fungi. In addition, it also acts as an indicator for peroxidase activity in fungi.
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Use of syringaldazine for detection of laccase in sporophores of wood rotting fungi
Harkin JM, et al.
Mycologia, 66(3), 469-476 (1974)
Aptitude of Oxidative Enzymes for Treatment of Wastewater Pollutants: A Laccase Perspective
Unuofin JO, et al.
Molecules (Basel), 24(11), 2064-2064 (2019)
Syringaldazine, an effective reagent for detecting laccase and peroxidase in fungi
Harkin JM and Obst JR
Experientia, 29(4), 381-387 (1973)