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Merck

SRE0026

Phosphatase substrate

Suitable for manufacturing of diagnostic kits and reagents

Synonym(s):

Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
NACRES:
NA.32
PubChem Substance ID:
329826114
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
MFCD00066288

Key Documents

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InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

assay

≥97% (HPLC)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

400

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Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBK7071V
SLBK7071V
SLCN6176
SLBT3157
SLBK7070V

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