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Merck

T3073

Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp™ Tissue Preparation Solution, REDExtract-N-Amp™ Tissue Preparation Solution, SYBR® Green Extract-N-Amp™ Tissue Preparation Solution

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About This Item

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55

Key Documents

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Quality Level

200

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp™ Tissue PCR kits, REDExtract-N-Amp™ Tissue PCR kits, and SYBR® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:
  • in combining segments of tails for genotyping
  • to extract genomic DNA from zebrafish embryos/fin tissues
  • to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
  • for extraction of DNA from fungal spores and mycelia

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000480820
0000480815
0000480815
0000402405
0000402404

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Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition
K D Kuhn et al.
Molecular and cellular neurosciences, 103, 103467-103467 (2020-02-01)
During neural development, complex organisms rely on progressive and regressive events whereby axons, synapses, and neurons are overproduced followed by selective elimination of a portion of these components. Tumor necrosis factor α (TNFα) together with its cognate receptor (Tumor necrosis

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