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About This Item
Clone:
T7 Tag
Technique(s):
western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase
Application:
WB CL
Citations:
9
isotype
IgM
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
T7 Tag
form
buffered aqueous solution
concentration
~2 mg/mL
technique(s)
western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Analysis Note
Antibody is suitable for ELISA and immunoblotting.
Application
Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.
General description
The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.
Immunogen
synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH
Physical form
Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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