04-953
Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
culture supernatant, clone A5S, Upstate®
Synonym(s):
Anti-Anti-CHTD8, Anti-Anti-JV18, Anti-Anti-JV18-1, Anti-Anti-LDS6, Anti-Anti-MADH2, Anti-Anti-MADR2, Anti-Anti-hMAD-2, Anti-Anti-hSMAD2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
A5S, monoclonal
Species reactivity:
human, zebrafish, mouse, canine
Application:
WB
Technique(s):
western blot: suitable
Citations:
30
Uniprot accession no.:
biological source
rabbit
antibody form
culture supernatant
antibody product type
primary antibodies
clone
A5S, monoclonal
species reactivity
human, zebrafish, mouse, canine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer465/pSer467)
Quality Level
Gene Information
human ... SMAD2(4087)
Biochem/physiol Actions
Predicted to cross-react with X. laevis based on sequence homology.
Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.
General description
55-60 kDa
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Analysis Note
Control
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
Routinely evaluated by immunoblot analysis.
Application
Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-953
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Linjia Jiang et al.
The Journal of experimental medicine, 215(5), 1337-1347 (2018-04-20)
Cell cycle quiescence is critical for hematopoietic stem cell (HSC) maintenance. TGF-β signaling in bone marrow niche has been identified in regulating HSC quiescence; however, the intrinsic regulatory mechanisms remain unclear. This study reports that Shp-1 knockout HSCs have attenuated
Oddrun Elise Olsen et al.
PloS one, 12(11), e0187349-e0187349 (2017-11-22)
Purified recombinant proteins for use in biomedical research are invaluable to investigate protein function. However, purity varies in protein batches made in mammalian expression systems, such as CHO-cells or HEK293-cells. This study points to caution while investigating effects of proteins
Amrita Chatterjee et al.
Cancer letters, 360(2), 134-140 (2015-02-11)
The mammalian target of rapamycin complex 1 (mTORC1) is a critical regulator of G1 cell cycle progression. Two key substrates of mTORC1 are ribosomal subunit S6 kinase (S6K) and eukaryotic initiation factor 4E (eIF4E) binding protein-1 (4E-BP1). We reported previously
Josephina A N Meester et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 19(4), 386-395 (2016-09-16)
Thoracic aortic aneurysm and dissection (TAAD) is typically inherited in an autosomal dominant manner, but rare X-linked families have been described. So far, the only known X-linked gene is FLNA, which is associated with the periventricular nodular heterotopia type of
Cathepsin K expression and activity in canine osteosarcoma.
J M Schmit,H C Pondenis,A M Barger,L B Borst,L D Garrett,J M Wypij,Z L Neumann,T M Fan
Journal of Veterinary Internal Medicine null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 04-953 | 04053252622533 |
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