556881
Ribonuclease Inhibitor, Human, Recombinant, E. coli
The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
form
liquid
specific activity
≥20,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
foreign activity
Endonuclease and RNase, none detected
shipped in
wet ice
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
Cell permeable: no
Primary Target
inactivates RNase
inactivates RNase
Product does not compete with ATP.
Reversible: no
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Other Notes
One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Physical form
In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.
Preparation Note
Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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