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Merck

77332

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

Synonym(s):

(2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase

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About This Item

CAS Number:
9003-99-0
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
MFCD00071339
EC Number:
1.11.1.7 (BRENDA, IUBMB)
Specific activity:
~150 U/mg
Biological source:
horseradish

Key Documents

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SMILES string

[O+H2]O[O-]

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

InChI

1S/H2O3/c1-3-2/h1-2H

biological source

horseradish

form

powder

quality

lyophilized

specific activity

~150 U/mg

Quality Level

100

solubility

50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)

shipped in

wet ice

storage temp.

2-8°C

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General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Application

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.
Peroxidase from horseradish has been used:
  • for isolation of N-Linked glycans
  • as a constituent of D-amino acid oxidase (DAAO) buffer
  • to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C
Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD8779
BCBV2718
BCBV3747
BCBP5939V

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J.A. Nicell et al.
Water Science and Technology, 25, 157-157 (1992)
Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans
Le HT, et al.
Journal of Analytical Methods in Chemistry, 2018 (2018)
Laura Alvarez et al.
Bio-protocol, 8(7), e2787-e2787 (2018-04-05)
Bacteria live in polymicrobial communities under tough competition. To persist in a specific niche many species produce toxic extracellular effectors as a strategy to interfere with the growth of nearby microbes. One of such effectors are the non-canonical D-amino acids.
Charles C Bell et al.
Nature communications, 10(1), 2723-2723 (2019-06-22)
Non-genetic drug resistance is increasingly recognised in various cancers. Molecular insights into this process are lacking and it is unknown whether stable non-genetic resistance can be overcome. Using single cell RNA-sequencing of paired drug naïve and resistant AML patient samples
G. Matheis et al.
Journal of Protein Chemistry, 3, 35-35 (1984)
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