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About This Item
Conjugate:
unconjugated
Technique(s):
indirect ELISA: 1:10,000
Application:
ELISA (i)
Citations:
10
biological source
mouse
conjugate
unconjugated
antibody product type
secondary antibodies
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:10,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
antibody form
purified from hybridoma cell culture
clone
KP-53
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General description
Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.
Application
Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
- immunodetection
- in western blot analysis
- in intracellular kappa light chain staining for flow cytometric analysis
Biochem/physiol Actions
The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes
McBRIDE OW, et al.
The Journal of Experimental Medicine, 155(5), 1480-1490 (1982)
Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
Zheng H, et al.
Molecular Immunology, 44(9), 2221-2227 (2007)
Heterogeneity of aberrant immunoglobulin expression in cancer cells
Hu D, et al.
Cellular & Molecular Immunology, 8(6), 479-479 (2011)
Chloe L Stoyle et al.
The Biochemical journal, 474(18), 3179-3188 (2017-08-09)
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence.
Megan D Lenardon et al.
Cell surface (Amsterdam, Netherlands), 6, 100047-100047 (2020-12-10)
Despite the importance of fungal cell walls as the principle determinant of fungal morphology and the defining element determining fungal interactions with other cells, few scalar models have been developed that reconcile chemical and microscopic attributes of its structure. The
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