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Merck

M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Synonym(s):

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

CAS Number:
9068-38-6
UNSPSC Code:
12352202
NACRES:
NA.55
MDL number:
MFCD00283754

Key Documents

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Product Name

M-MLV Reverse Transcriptase, Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

biological source

Porcine intestinal mucosa

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 200 reactions

feature

dNTPs included: no
hotstart: no

concentration

200 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

M-MLV Reverse Transcriptase has been used:
  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • in quantitative realtime-polymerase chain reaction (RT-qPCR)
  • in reverse transcription

Features and Benefits

  • Thermostable reverse transcriptase active at 37 °C.
  • Can be used to generate first strand cDNA of up to 7 kb.

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Other Notes

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000472176
0000472176
0000401710
0000401710
0000279806

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Dikaios I, et al.
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Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora A, TRKB and affects proteins involved in apoptosis and autophagy pathways
Durbas M, et al.
International Journal of Oncology, 49(2), 823-837 (2016)
Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora A, TRKB and affects proteins involved in apoptosis and autophagy pathways
Durbas M, et al.
International Journal of Oncology, 49, 823-837 (2016)
Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase
Cote ML and Roth MJ
Virus Research, 134, 186-202 (2008)
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