S6639
SSC Buffer 20× Concentrate
for Northern and Southern blotting, solution
Synonym(s):
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105319
grade
Molecular Biology
Quality Level
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Application
SSC Buffer 20× Concentrate has been used
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component