S8398
Shields and Sang M3 Insect Medium
With L-glutamine, without potassium bicarbonate, powder, suitable for insect cell culture
form
powder
technique(s)
cell culture | insect: suitable
components
NaHCO3: no
L-glutamine: 0.6 g/L
glucose: 10 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
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Application
Developed for the culture of Drosophila cell lines.
Shields and Sang M3 Insect Medium has been used:
- for the dissection of Drosophila larvae to study the changes in the translational activity of larval wing imaginal disc cells
- to incubate Drosophila bodies for surface sensing of translation (SUnSET) assay
- to culture Drosophila cells for transfection studies
General description
Shields and Sang M3 insect medium can support the rapid growth of both primary and established cultures of Drosophila melanogaster cells. Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Alind Gupta et al.
Journal of cell science, 131(16) (2018-07-29)
Cilia are cellular antennae that are essential for human development and physiology. A large number of genetic disorders linked to cilium dysfunction are associated with proteins that localize to the ciliary transition zone (TZ), a structure at the base of
Yuankai Dong et al.
Genetics, 215(1), 89-101 (2020-03-18)
BEAF (Boundary Element-Associated Factor) was originally identified as a Drosophila melanogaster chromatin domain insulator-binding protein, suggesting a role in gene regulation through chromatin organization and dynamics. Genome-wide mapping found that BEAF usually binds near transcription start sites, often of housekeeping
Fabien Cannac et al.
Science advances, 6(16), eaay7928-eaay7928 (2020-06-05)
The Hedgehog (Hh) signaling pathway controls embryonic development and adult tissue homeostasis in multicellular organisms. In Drosophila melanogaster, the pathway is primed by secretion of a dually lipid-modified morphogen, Hh, a process dependent on a membrane-integral protein Dispatched. Although Dispatched
Silke Rothenbusch-Fender et al.
Biology open, 6(12), 1876-1888 (2017-11-11)
During
Tim Hundertmark et al.
PloS one, 13(9), e0203622-e0203622 (2018-09-08)
Spermatogenesis in many species including Drosophila melanogaster is accompanied by major reorganisation of chromatin in post-meiotic stages, involving a nearly genome-wide displacement of histones by protamines, Mst77F and Protamine-like 99C. A proposed prerequisite for the histone-to-protamine transition is massive histone
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