Skip to Content
Merck

Key Documents

View All Documentation

389561

Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

Synonym(s):

Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
37326-33-3
EC Number:
3.2.1.35 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00131351
Specific activity:
≥2000 units (A280)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

conjugate

conjugate (Glucosaminoglycan)

Quality Level

100

description

Merck USA index - 14, 4758

form

lyophilized powder

specific activity

≥2000 units (A280)

packaging

ampule of ≥100 U (1 U = 1 TRU (Turbidity Reducing Unit))

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

0.5 M acetate, pH 6.0: 30 U/mL, water: 30 U/mL

foreign activity

protease ≤0.05 U/ampule (pH 6.0)

shipped in

ambient

storage temp.

2-8°C

General description

Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.



Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.

Other Notes

Baker, J.R., et al. 2002. Biochem. J.365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
One TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Still not finding the right product?

Explore all of our products under Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jennifer E Rowley et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(1), 75-91 (2019-11-13)
Hyaluronan (HA) is a ubiquitous component of the extracellular matrix. The spatial-temporal localization of HA can be visualized in situ using biotinylated HA binding proteins (HABPs). This assay is sensitive to fixation conditions, and there are currently no best practices